Binary Ethylenimine Inactivated Japanese Encephalitis Virus Antigen Reveals Hemagglutination

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Binary Ethylenimine Inactivated Japanese Encephalitis Virus Antigen Reveals Hemagglutination

Severe climate change and global warming may impact significantly on vector-borne disease including Japanese encephalitis (JE) infection in human and animals. Thus, veterinary authority requires large quantity of diagnostic tools to survey vector-borne diseases. New producing method having a relation with JE antigen is needed to substitute conventional sucrose-acetone extraction method using su...

متن کامل

Key words: ELISA IgM titer, Hemagglutination inhibition, Japanese encephalitis virus antibodies, Japanese encephalitis epidemics in pigs

特 定飼 育 ブ タ10頭 につ いて,個 々 に血 液 を採取 し抗 体価 を測 定 した ところ,流 行前 期 にはELISAIgM 抗 体価 は103以 下で,HI抗 体 は検 出 され なか った.HI法 に よ るIgM抗 体 が現 われ始 め ると,ELISAIgM 抗体価 は104以 上 を示 した.ELISAIgM抗 体 は約2週 間 継続 して検 出 され た.そ の後ELISAIgM抗 体 価 は103前 後 に低 下 した が,HI反 応 は陽性 で あ り,ほ とん どIgG抗 体 であ った. と畜場 ブタ につ い ては,定 期 的 に20頭 ず つ検査 し,非 流 行期 及 び流行前 期 のELISAIgM抗 体 価 は103 以下 で あ った.流 行期 に入 る とHI法 に よるIgM抗 体 は陽性 とな り,そ の ほ とん どがELISAIgM...

متن کامل

Rabies virus inactivation by binary ethylenimine: new method for inactivated vaccine production.

The inactivation dynamics of rabies virus (PV strain) by binary ethylenimine, and the immunogenic properites and the stability of the vaccines prepared using this agent, were studied. Binary ethylenimine at a final concentration of 0.01 M was prepared wtih 2-bromoethylamine hydrobromide in alkaline solutions, either separately from or in suspensions of rabies virus propagated in BHK cells. The ...

متن کامل

Immunogenicity of commercial, formaldehyde and binary ethylenimine inactivated Newcastle disease virus vaccines in specific pathogen free chickens

Newcastle disease (ND) is one of the most important diseases that affect birds; the epizootic nature of the disease has caused severe economic losses in the poultry industry worldwide. In this experiment ND virus (NDV) was inactivated by two different chemicals binary ethylenimine (BEI) and formaldehyde. Formaldehyde was used at 0.1%, while BEI was used at concentrations of 1 to 4 mM. NDV inact...

متن کامل

Immune Response to Inactivated Newcastle Disease Virus by Electrolysed Catholyte Anolyte and Binary Ethylenimine in Specific Pathogen Free Chickens

This study aimed to investigate the immunogenicity of Newcastle disease virus (NDV) in specificpathogen-free (SPF) chickens. The NDV was inactivated using either Binary ethyleneimine (BEI) or Electrolysed water-Catholyte-Anolyte (ECA). Complete inactivation of NDV occurred after 24 hours with either BEI or ECA. Prepared inactivated NDV vaccines were tested for their efficiency in generating hum...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Open Journal of Veterinary Medicine

سال: 2012

ISSN: 2165-3356,2165-3364

DOI: 10.4236/ojvm.2012.23020