Binding of terbium and cisplatin to C13* human ovarian cancer cells using time-resolved terbium luminescence
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چکیده
منابع مشابه
Determination of the Binding Constant of Terbium-Transferrin
Apotransferrin (apo Tf) in 0.1 M N-(2hydroxyethyl)piperazine-N2-ethanesulfanic acid at 25 ˚C and pH 7.4 has been titrated with acidic solution of Tb3+. The binding of Tb3+ at the two specific metal-binding sites of transferrin was followed from the changes in the difference UV spectra at 245 nm. The molar absorptivity per binding site for Tb3+...
متن کاملDevelopment and Validation of a Terbium-Sensitized Luminescence Analytical Method for Deferiprone
A sensitive fluorometric method for the determination of deferiprone (DFP) based on the formation of a luminescent complex with Tb3+ ions in aqueous solutions is reported. The maximum excitation and emission wavelengths were 295 and 545 nm, respectively. The effects of various factors on the luminescence intensity of the system were investigated and optimized, then under the optimum conditions,...
متن کاملDevelopment and Validation of a Terbium-Sensitized Luminescence Analytical Method for Deferiprone
A sensitive fluorometric method for the determination of deferiprone (DFP) based on the formation of a luminescent complex with Tb3+ ions in aqueous solutions is reported. The maximum excitation and emission wavelengths were 295 and 545 nm, respectively. The effects of various factors on the luminescence intensity of the system were investigated and optimized, then under the optimum conditions,...
متن کاملSynthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay.
A new nonadentate ligand, N, N, N1, N1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-phenylpyridine]tetrakis(acetic acid) (BPTA) for a Tb3+ fluorescent complex was synthesized. The Tb3+ complex is strongly fluorescent, having a large fluorescence quantum yield of 1.00 and very long fluorescence lifetime of 2.681 ms in 0.05 M borate buffer of pH 9.1. Streptavidin (SA) was labeled with BPTA by using it...
متن کاملEngineering a terbium-binding site into an integral membrane protein for luminescence energy transfer.
Luminescence resonance energy transfer with a lanthanide like Tb(3+) as donor is a useful technique for estimating intra- and intermolecular distances in macromolecules. However, the technique usually requires the use of a bulky chelator with a flexible linker attached to a Cys residue to bind Tb(3+) and, for intramolecular studies, an acceptor fluorophor attached to another Cys residue in the ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
سال: 1998
ISSN: 0167-4889
DOI: 10.1016/s0167-4889(98)00127-x