Binding parameters of antibodies: pseudo-affinity and other misconceptions
نویسندگان
چکیده
منابع مشابه
Binding Affinity of Antibodies?
Although the switch process is frequently associated with affinity maturation, the constant region is not assumed to play a role in Ag–Ab binding. In the present work, we demonstrate that two clonally related human monoclonal Igs sharing identical V H and V L sequences, but expressing different isotypes (IgA1 k PER and IgG1 k PER ), bind tubulin with significantly different affinities. This dif...
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15 صفحه اولMeasurement of Affinity Constant of Anti-human IgG Monoclonal Antibodies by an ELISA-based Method
Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...
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ژورنال
عنوان ژورنال: Cancer Immunology, Immunotherapy
سال: 2005
ISSN: 0340-7004,1432-0851
DOI: 10.1007/s00262-005-0046-1