Bioaerosol sampling for the detection of aerosolized influenza virus

Bioaerosol sampling for the detection of aerosolized influenza virus

BACKGROUND Influenza virus was used to characterize the efficacy of a cyclone-based, two-stage personal bioaerosol sampler for the collection and size fractionation of aerosolized viral particles. METHODS A Collison single-jet nebulizer was used to aerosolize the attenuated FluMist vaccine into a calm-air settling chamber. Viral particles were captured with bioaerosol samplers that utilize 2 ...

Immunofluorescence Method for The Detection of Non-Pathogenic Influenza Virus

The direct F.A. Method has been used for diffe­rentiation of virulent and avirulent lines of influenza viruses. A positive fluorescence was obtained indi­cating the level of virus in the tissues, sorbing with dried mouse powder helped to reduce non-specific staining. The virulent line showed the greatest num­ber of fluorescent cells with the peak at 10 hours. the non-virulent lines showed a gra...

Standardization of ELISA for Detection of Avian Influenza Virus Antibodies

Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles

The development and characterization of a quartz crystal microbalance (QCM) sensor for the direct detection of aerosolized influenza A virions s reported. Self-assembled monolayers (SAMs) of mercaptoundecanoic acid (MUA) are formed on QCM gold electrodes to provide a surface menable for the immobilization of anti-influenza A antibodies using NHS/EDC coupling chemistry. The surface-bound antibod...

Evaluation of bioaerosol sampling techniques for the detection of in contaminated air

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