Bioanalytical Method Development And Validation Of Darunavir In Biological Matrices Using Uplc-Ms-Ms
نویسندگان
چکیده
Background: To develop a simple and accurate method for the determination of Darunavir in human plasma by liquid-liquid extraction with Verapamil as an internal standard using UPLC-MS/MS. Methods: The separation was achieved on Agilent, Zorbax XDB C18 (2.1 x 50 mm ID, 5 μm) column. samples were chromatographed mobile phase consisting acetonitrile 2mM ammonium formate 0.1% formic acid water (70:30 v/v) at flow rate 0.12 ml/min mass spectrometer ESI chamber. eluted 1.35 1.13 min respectively total run time 3 min. Results: A linear response established 10- 2,000 ng/ml correlation coefficient 0.9919. % accuracy found to be 98% 102.40%. rapid developed plasma. strictly validated according ICH guidelines.
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical Negative Results
سال: 2022
ISSN: ['0976-9234', '2229-7723']
DOI: https://doi.org/10.47750/pnr.2022.13.s09.615