Biofilm microbiome in extracorporeal membrane oxygenator catheters

Despite the formation of biofilms on catheters for extracorporeal membrane oxygenation (ECMO), some patients do not show bacteremia. To elucidate specific linkage between and bacteremia in with ECMO, an improved understanding microbial community within catheter is necessary. Hence, we aimed to evaluate biofilm microbiome ECMO from adults (n = 6) without 15) The microbiomes were evaluated by profiling V3 V4 regions bacterial 16s rRNA genes using Illumina MiSeq sequencing platform. In total, 2,548,172 reads, average 121,341 reads per sample, generated. Although alpha diversity was slightly higher non-bacteremic group, difference statistically significant. addition, there no beta two groups. We found 367 different genera, which 8 present all samples regardless group; Limnohabitans , Flavobacterium Delftia Massilia Bacillus Candidatus Xiphinematobacter CL0-1 showed abundance more than 1% sample. particular, Arthrobacter SMB53 Neisseria Ortrobactrum Rhabdochlamydia Deefgae Dyella Paracoccus Pedobacter highly abundant bacteremic group. Network analysis indicated that group complex CL0 . 1 considered important genera because they connected subnetworks. Biofilm characteristics varied according presence or absence There significant differences groups, but composition biofilms. biofilm-associated dynamic, showing very network connections microbiome.