Biological and Biochemical Characterization of Macrophage Activating Factor (MAF) in Murine Lymphocytes: Physicochemical Similarity of MAF to Gamma Interferon (IFN-γ)
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چکیده
منابع مشابه
Biological and antigenic similarities of murine interferon-gamma and macrophage-activating factor
Murine peritoneal exudate cells (PEC) treated with murine recombinant interferon-gamma (IFN-gamma) (greater than 99% estimated purity), or concanavalin A-stimulated spleen cell supernatants developed tumoricidal properties (macrophage activation factor [MAF] activity). MAF activity was found to occur with treatments of 10 U/ml IFN-gamma, and at levels as low as 1 U/ml IFN-gamma if a second sign...
متن کاملAssociation of the macrophage activating factor (MAF) precursor activity with polymorphism in vitamin D-binding protein.
BACKGROUND Serum vitamin D-binding protein (Gc protein or DBP) is a highly expressed polymorphic protein, which is a precursor of the inflammation-primed macrophage activating factor, GcMAF, by a cascade of carbohydrate processing reactions. In order to elucidate the relationship between Gc polymorphism and GcMAF precursor activity, we estimated the phagocytic ability of three homotypes of Gc p...
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We have isolated a selectively deglycosylated form of vitamin D binding protein (DBP-maf) generated from systemically available DBP by a human pancreatic cancer cell line. DBP-maf is antiproliferative for endothelial cells and antiangiogenic in the chorioallantoic membrane assay. DBP-maf administered daily was able to potently inhibit the growth of human pancreatic cancer in immune compromised ...
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The vitamin D-binding protein, also known as groupspecific component (Gc) or Gc-globulin, is a 51.2-kDa polymorphic protein of the a2-macroglobulin fraction of human plasma (1 ). In human population, three common alleles (Gc1f, Gc1s, and Gc2) and .120 rare variant alleles have been classified by isoelectric focusing (2 ). Gc1f and Gc1s contain sialic acid residues, whereas Gc2 does not (2 ). Gc...
متن کاملEvidence for a gamma-interferon receptor that regulates macrophage tumoricidal activity
Gamma-interferon (IFN-gamma) is the macrophage-activating factor (MAF) produced by normal murine splenic cells and the murine T cell hybridoma 24/G1 that induces nonspecific tumoricidal activity in macrophages. Incubation of 24/G1 supernatants diluted to 8.3 IRU IFN-gamma/ml with 6 X 10(6) elicited peritoneal macrophages or bone marrow-derived macrophages for 4 h at 37 degrees C, resulted in re...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1984
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1984.tb00723.x