Biological and functional properties of human umbilical cord-derived lyophilized tissue-engineered matrices

The use of tissue-engineered products (TEP) from decellularized extracellular matrix (dECM) to treat deep skin lesions is a tissue engineering method that promotes regenerative healing. Cell-free preparations reproduce the hierarchical complexity tissues, mimic structural, biochemical and mechanical signals are necessary attract cells, source bioactive molecules. human umbilical cord biomaterial has fetal phenotype with extra-embryonic origin, therefore available no ethical limitations in its use. laboratory at Kirov Military Medical Academy developed patented TEP highly form hydrogel matrix. To study potential, lyophilisates solid-state matrices were implanted around mini pig fullthickness wounds vivo. external signs inflammatory response histological images biopsy specimens lyophilizate implantation areas analyzed. effect nutrient media, «conditioned» lyophilizates both matrices, on viability migration activity fibroblast-like isolated skin, was investigated. showed good biocompatibility bioactivity vitro vivo experiments. Implantation samples promoted faster formation mature epidermis compared control.