Biosynthesis, import and processing of precursor polypeptides of mammalian mitochondrial pyruvate dehydrogenase complex
نویسندگان
چکیده
منابع مشابه
Biosynthesis, import and processing of precursor polypeptides of mammalian mitochondrial pyruvate dehydrogenase complex.
An immunological analysis has been conducted of early events in the biosynthesis, import and assembly of the mammalian pyruvate dehydrogenase complex (PDC). For this purpose, monospecific polyclonal antisera were produced against the intact assembly from ox heart, Mr 8.5 x 10(6), and each of its component polypeptides, E1 alpha, E1 beta, E2, E3 and protein X. Optimal detergent-based incubation ...
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Rat liver total RNA was translated in a reticulocyte lysate, and the precursor of rat liver mitochondrial malate dehydrogenase was identified by a monospecific antibody against the denatured mature enzyme. The precursor is about Mr = 1500 to 2000 larger than the monomeric form of the mature protein. The major spots of the two-dimensional peptide map of the two proteins were identical. The precu...
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We have presented evidence that stimulation of pyruvate dehydrogenase, (PDH.) kinase activity by pyruvate or by acetyl-CoA is mediated through acetylation of lipoyl moieties (Cate, R. L., and Roche, T. E. (1978) J. Biol. Chem. 253,496-503). In accord with this indirect mechanism for the action of these effecters, we now find that the degree of stimulation of PDH, kinase increases with the level...
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We have presented evidence that stimulation of pyruvate dehydrogenase, (PDH.) kinase activity by pyruvate or by acetyl-CoA is mediated through acetylation of lipoyl moieties (Cate, R. L., and Roche, T. E. (1978) J. Biol. Chem. 253,496-503). In accord with this indirect mechanism for the action of these effecters, we now find that the degree of stimulation of PDH, kinase increases with the level...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1988
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2510817