Butanediol Conversion to Gamma‐Hydroxybutyrate Markedly Reduced by the Alcohol Dehydrogenase Blocker Fomepizole
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چکیده
منابع مشابه
Improved Production of 2,3-Butanediol in Bacillus amyloliquefaciens by Over-Expression of Glyceraldehyde-3-Phosphate Dehydrogenase and 2,3-butanediol Dehydrogenase
BACKGROUND Previously, a safe strain, Bacillus amyloliquefaciens B10-127 was identified as an excellent candidate for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD). However, B. amyloliquefaciens fermentation yields large quantities of acetoin, lactate and succinate as by-products, and the 2,3-BD yield remains prohibitively low for commercial production. METHODOLOGY/PRINCI...
متن کاملReconstruction of an acetogenic 2,3-butanediol pathway involving a novel NADPH-dependent primary-secondary alcohol dehydrogenase.
Acetogenic bacteria use CO and/or CO2 plus H2 as their sole carbon and energy sources. Fermentation processes with these organisms hold promise for producing chemicals and biofuels from abundant waste gas feedstocks while simultaneously reducing industrial greenhouse gas emissions. The acetogen Clostridium autoethanogenum is known to synthesize the pyruvate-derived metabolites lactate and 2,3-b...
متن کاملCyanobacterial conversion of carbon dioxide to 2,3-butanediol.
Conversion of CO(2) for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically developed the 2,3-butanediol (23BD) biosynthetic pathway in Synechococcus elongatus PCC7942 as...
متن کاملThe short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde.
Mutants able to germinate and perform early growth in medium containing a high NaCl concentration were identified during the course of two independent screenings and named salt resistant (sre) and salobreño (sañ). The sre and sañ mutants also were able to germinate in high-osmoticum medium, indicating that they are osmotolerant in a germination assay. Complementation analyses revealed that sre1...
متن کاملSpecific Blood Alcohol Measurements on the Autoanalyzer by Alcohol Dehydrogenase
Introduction m a n y a c c e p t e d chemical methods for blood alcohol involve the oxidation of alcohol after a separation step1’ 2 and the subsequent determination of the excess of oxidant. On the other hand, enzymatic procedures1-4 are based on the conversion of diphosphopyridine nucleotide (DPN) to its reduced form (DPNH), catalysed by alcohol dehydrogenase (ADH) in the presence of ethyl-al...
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ژورنال
عنوان ژورنال: Clinical Pharmacology & Therapeutics
سال: 2019
ISSN: 0009-9236,1532-6535
DOI: 10.1002/cpt.1306