CACA-TOCSY with alternate 13C–12C labeling: a 13Cα direct detection experiment for mainchain resonance assignment, dihedral angle information, and amino acid type identification
نویسندگان
چکیده
منابع مشابه
HNCA-TOCSY-CANH experiments with alternate (13)C- (12)C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment.
Described here is a set of three-dimensional (3D) NMR experiments that rely on CACA-TOCSY magnetization transfer via the weak ³J(CαCα) coupling. These pulse sequences, which resemble recently described (13)C detected CACA-TOCSY (Takeuchi et al. 2010) experiments, are recorded in (1)H(2)O, and use (1)H excitation and detection. These experiments require alternate (13)C-(12)C labeling together wi...
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One bottleneck in NMR structure determination lies in the laborious and time-consuming process of side-chain resonance and NOE assignments. Compared to the well-studied backbone resonance assignment problem, automated side-chain resonance and NOE assignments are relatively less explored. Most NOE assignment algorithms require nearly complete side-chain resonance assignments from a series of thr...
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ژورنال
عنوان ژورنال: Journal of Biomolecular NMR
سال: 2010
ISSN: 0925-2738,1573-5001
DOI: 10.1007/s10858-010-9410-3