Can Next-Generation Sequencing Replace Sanger Sequencing for Screening Genetic Variants?
نویسندگان
چکیده
منابع مشابه
Targeted next-generation sequencing can replace Sanger sequencing in clinical diagnostics.
Mutation detection through exome sequencing allows simultaneous analysis of all coding sequences of genes. However, it cannot yet replace Sanger sequencing (SS) in diagnostics because of incomplete representation and coverage of exons leading to missing clinically relevant mutations. Targeted next-generation sequencing (NGS), in which a selected fraction of genes is sequenced, may circumvent th...
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ژورنال
عنوان ژورنال: Circulation Journal
سال: 2014
ISSN: 1346-9843,1347-4820
DOI: 10.1253/circj.cj-14-1144