Capturing Conformational States in Proteins Using Sparse Paramagnetic NMR Data

Capturing Conformational States in Proteins Using Sparse Paramagnetic NMR Data

Capturing conformational changes in proteins or protein-protein complexes is a challenge for both experimentalists and computational biologists. Solution nuclear magnetic resonance (NMR) is unique in that it permits structural studies of proteins under greatly varying conditions, and thus allows us to monitor induced structural changes. Paramagnetic effects are increasingly used to study protei...

NMR of paramagnetic proteins

Ligand-driven conformational changes of MurD visualized by paramagnetic NMR.

Proteins, especially multi-domain proteins, often undergo drastic conformational changes upon binding to ligands or by post-translational modifications, which is a key step to regulate their function. However, the detailed mechanisms of such dynamic regulation of the functional processes are poorly understood because of the lack of an efficient tool. We here demonstrate detailed characterizatio...

Structural studies of proteins by paramagnetic solid-state NMR spectroscopy.

Paramagnetism-based nuclear pseudocontact shifts and spin relaxation enhancements contain a wealth of information in solid-state NMR spectra about electron-nucleus distances on the ∼20 Å length scale, far beyond that normally probed through measurements of nuclear dipolar couplings. Such data are especially vital in the context of structural studies of proteins and other biological molecules th...

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فعالیت های نگهداری و تعمیرات اطلاعاتی را تولید می کند که می تواند در تعیین زمان های بیکاری و ارایه یک برنامه زمان بندی شده یا تعیین هشدارهای خرابی به پرسنل نگهداری و تعمیرات کمک کند. وقتی که مقدار داده های تولید شده زیاد باشند، فهم بین متغیرها بسیار مشکل می شوند. این پایان نامه به کاربردی از داده کاوی برای کاوش پایگاه های داده چندبعدی در حوزه نگهداری و تعمیرات، برای پیدا کردن خرابی هایی که موجب...