Carbohydrate Microarray on Glass: A Tool for Carbohydrate-Lectin Interactions

Carbohydrate Microarray

Synthetic Approaches to Study Multivalent Carbohydrate–Lectin Interactions

The specific recognition of carbohydrate structures in biological systems (Box 5) by carbohydrate-binding proteins (lectins) is the basis of numerous intraand intercellular events ranging from the control of protein folding to cell–cell communication during development, inflammation, and cancer metastasis [1]. Investigation of carbohydrate–lectin interactions can be approached from two directio...

Probing multivalent carbohydrate-lectin interactions by an enzyme-linked lectin assay employing covalently immobilized carbohydrates.

We report here the synthesis of a series of mono- to trivalent N-acetylglucosamine (GlcNAc) derivatives as ligands for the plant lectin wheat germ agglutinin (WGA). Their WGA binding potencies were determined by an established enzyme-linked lectin assay (ELLA) employing microtiter plates with non-covalently immobilized porcine stomach mucin (PSM) as reference ligand and an ELLA with a new GlcNA...

Carbohydrate-aromatic interactions.

The recognition of saccharides by proteins has far reaching implications in biology, technology, and drug design. Within the past two decades, researchers have directed considerable effort toward a detailed understanding of these processes. Early crystallographic studies revealed, not surprisingly, that hydrogen-bonding interactions are usually involved in carbohydrate recognition. But less exp...

Boron-Carbohydrate Interactions

Boron-polyol interactions are of fundamental importance to human health [1], plant growth [2] and quorum sensing among certain bacteria [3]. Such diversity is perhaps not surprising when one considers boron is one of the ten most abundant elements in sea water and carbohydrates make up the planet’s most abundant class of biomass. Several boronic acids matrices are commercially available for the...