Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast

High-resolution Live Cell Imaging of the Yeast Life Cycle

Layout, operation, and results of a microfluidic device, which allows for anaerobic and aerobic cultivation of yeast cells over extended time (>3 days) are presented. The use of this device in combination with automated image analysis software and multiple fluorescence reporters (>4 fluorescence protein per strain) allows for studying timing and coherence of morphological features, the cell-cyc...

Live In-Cell Visualization of Proteins Using Super Resolution Imaging

Optical diffraction tomography for high resolution live cell imaging Citation

We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we ap...

Optical diffraction tomography for high resolution live cell imaging.

We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we ap...

High-content super-resolution imaging of live cell by uPAINT.

In this chapter, we present the uPAINT method (Universal Point Accumulation Imaging in Nanoscale Topography), a simple single-molecule super-resolution method which can be implemented on any wide field fluorescence microscope operating in oblique illumination. The key feature of uPAINT lies in recording high numbers of single molecules at the surface of a cell by constantly labeling while imagi...