Cell age dependent concentration of Escherichia coli divisome proteins analyzed with ImageJ and ObjectJ

Cell age dependent concentration of Escherichia coli divisome proteins analyzed with ImageJ and ObjectJ

The rod-shaped Gram-negative bacterium Escherichia coli multiplies by elongation followed by binary fission. Longitudinal growth of the cell envelope and synthesis of the new poles are organized by two protein complexes called elongasome and divisome, respectively. We have analyzed the spatio-temporal localization patterns of many of these morphogenetic proteins by immunolabeling the wild type ...

Assembly and activation of the Escherichia coli divisome.

Cell division in Escherichia coli is mediated by a large protein complex called the divisome. Most of the divisome proteins have been identified, but how they assemble onto the Z ring scaffold to form the divisome and work together to synthesize the septum is not well understood. In this review, we summarize the latest findings on divisome assembly and activation as well as provide our perspect...

Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli.

We describe here details of the method we used to identify and distinguish essential from nonessential genes on the bacterial Escherichia coli chromosome. Three key features characterize our method: high-efficiency recombination, precise replacement of just the open reading frame of a chromosomal gene, and the presence of naturally occurring duplications within the bacterial genome. We targeted...

Plasmonic Sensors for Identification and Determination of Escherichia Coli Pathogenic bacterial concentration

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Binding Proteins to Maintenance of Cell Shape in Escherichia coli