Cell-Based Fuzzy Metrics Enhance High-Content Screening (HCS) Assay Robustness

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Methods for High-Content, High-Throughput Image-Based Cell Screening

Visual inspection of cells is a fundamental tool for discovery in biological science. Modern robotic microscopes are able to capture thousands of images from massively parallel experiments such as RNA interference (RNAi) or small-molecule screens. Such screens also benefit from lab automation, making large screens, e.g., genome-scale knockdown experiments, more feasible and common. As such, the...

متن کامل

Microscopy-Based High-Content Screening

Image-based screening is used to measure a variety of phenotypes in cells and whole organisms. Combined with perturbations such as RNA interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. Screens have been applied to study diverse processes, such as protein-localization changes, cancer cell vulnerabilities, a...

متن کامل

Systems cell biology based on high-content screening.

A new discipline of biology has emerged since 2004, which we call "systems cell biology" (SCB). Systems cell biology is the study of the living cell, the basic unit of life, an integrated and interacting network of genes, proteins, and myriad metabolic reactions that give rise to function. SCB takes advantage of high-content screening platforms, but delivers more detailed profiles of cellular s...

متن کامل

Composite primary neuronal high-content screening assay for Huntington's disease incorporating non-cell-autonomous interactions.

Huntington's disease (HD) is a fatal neurodegenerative disease characterized by progressive cognitive, behavioral, and motor deficits and caused by expansion of a polyglutamine repeat in the Huntingtin protein (Htt). Despite its monogenic nature, HD pathogenesis includes obligatory non-cell-autonomous pathways involving both the cortex and the striatum, and therefore effective recapitulation of...

متن کامل

High-content Screening Assay for Huntington’s disease Incorporating non-cell-Autonomous Interactions

Huntington’s disease (Hd) is a fatal, genetic neurodegenerative disease characterized by progressive cognitive, behavioral, and motor deficits over a period of 15 to 25 years leading to disability and death. limited palliative treatments for Hd are currently available but there is no effective cure; therefore there is a significant unmet need for developing drug therapies that can modify the di...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Biomolecular Screening

سال: 2013

ISSN: 1087-0571,1552-454X

DOI: 10.1177/1087057113501554