Cell Death Caused by Single-Stranded Oligodeoxynucleotide-Mediated Targeted Genomic Sequence Modification
نویسندگان
چکیده
منابع مشابه
Cell death caused by single-stranded oligodeoxynucleotide-mediated targeted genomic sequence modification.
Targeted gene repair directed by single-stranded oligodeoxynucleotides (ssODNs) offers a promising tool for biotechnology and gene therapy. However, the methodology is currently limited by its low frequency of repair events, variability, and low viability of "corrected" cells. In this study, we showed that during ssODN-mediated gene repair reaction, a significant population of corrected cells f...
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The development of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) facilitates in vitro studies of human disease mechanisms, speeds up the process of drug screening, and raises the feasibility of using cell replacement therapy in clinics. However, the study of genotype-phenotype relationships in ESCs or iPSCs is hampered by the low efficiency of site-specific gene e...
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ژورنال
عنوان ژورنال: Oligonucleotides
سال: 2009
ISSN: 1545-4576,1557-8526
DOI: 10.1089/oli.2009.0191