Cellular Uptake and Localization of Hematoporphyrin Derivatives in Lung Adenocarcinoma A549, Squamous Carcinoma H520 and Small Cell Carcinoma H446 Cell Lines

To select bronchial epithelial BEAS-2B cell line as control, cellular uptake and localization of Hematoporphyrin derivatives (HPD) were compared between normal cells (BEAS-2B), various lung cancer lines, well lines. Cellular HPD was quantified by fluorescence intensity with a multifunctional microplate reader flow cytometry. Laser scanning confocal microscopy used to observe compare the distribution HPD. In certain concentration range, positively correlated intensity. increased over time in four The average significantly different (P<0.0001). lines showed red fluorescent signals varying intensities, all distributed cytoplasm. vitro experiments show that there are differences lines: ability take A549 is strongest, followed H446 cells, H520 weakest. photosensitizer located cytoplasm both showing punctate distribution.