Cellulose Acetate Electrophoresis of Milk Serum Proteins
نویسندگان
چکیده
منابع مشابه
Rapid cellulose acetate electrophoresis. I. Serum proteins.
A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...
متن کاملEstimation of serum proteins by electrophoresis on cellulose acetate.
We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained .albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) X total serum protein concn] and by the formula (absorbance of albumin-bou...
متن کاملSerum Lipoproteins in Multiple Sclerosis Determined by Cellulose Acetate Electrophoresis.
In previous papers (Gavrilescu, Jellinek, and McMenemey, 1961, Gavrilescu and McMenemey, 1964) on comparative studies on the protein fractions in the serum and cerebrospinal fluid in multiple sclerosis we have noted increases in the °C2 and f globulins in the serum. Although not specific for this disease these changes are of interest, particularly as multiple sclerosis is considered to be purel...
متن کاملUnderestimation of monoclonal proteins by serum protein electrophoresis on cellulose acetate.
Our study of 95 serum samples from 37 patients with monoclonal gammopathy revealed distorted irregular monoclonal (M) protein bands after serum protein electrophoresis (SPE) on cellulose acetate membrane. In 71 (75%) of the 95 sera, the M-protein was underestimated and the albumin concentration overestimated. Dilution of the serum sample before SPE eliminated the abnormality of the M-protein ba...
متن کاملTwo new non-barbiturate buffers for electrophoresis of serum proteins on cellulose acetate membranes.
Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek" system. One buffer is a tris(hydroxymethyl)aminomethane (Tris)/hippurate formulation; the other is composed of Tris/N-[tris(h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1962
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(62)89477-6