Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21

Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli

Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY...

Advances and challenges in recombinant protein expression in Escherichia coli

comparison of the extracellular full-length and truncated recombinant protein a production in escherichia coli bl21 (de3)

protein a is a commercially important protein in biotechnological and medicinal applications. the great value of this protein and its applications in genetic and protein engineering and microbial researches as well as the growing use in biochemical industries, biotechnology, medicine and pharmacology, highlight the importance of the present study. in this survey the encoding genes of full-lengt...

Fuzzy Hybrid least-Squares Regression Approach to Estimating the amount of Extra Cellular Recombinant Protein A from Escherichia coli BL21

Introduction: Immune Protein A is a component with a vast spectrum of biochemical, biological and medical usages. The coding gene of this protein was extracted from Staphylococcus aureus and was cloned and expressed in Escherichia coli bacteria. Suitable statistical methods are utilized to optimize expression conditions  for evaluating experiment accuracy , guarantee the accuracy of subsequent ...

Recovery of recombinant bromelain from Escherichia coli BL21-AI

In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombina...