Changes in Microtubule Protofilament Number Induced by Taxol Binding to an Easily Accessible Site
نویسندگان
چکیده
منابع مشابه
Taxol influences control of protofilament number at microtubule-nucleating sites in Drosophila.
Control of protofilament number has been investigated using Drosophila wings at a stage when 15-protofilament microtubules assemble under normal conditions. Microtubule nucleation still progressed at the usual microtubule-nucleating sites in the presence of taxol. However, provided taxol was introduced before microtubule nucleation began, few microtubules with 15 protofilaments assembled. Most ...
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The synchrotron x-ray solution scattering profiles of microtubules assembled from purified GDP- or GTP-tubulin with the antitumor drug docetaxel (Taxotere) are consistent with identical non-globular alpha and beta-tubulin monomers ordered within the known surface lattice of microtubules, with a center to center lateral spacing of 5.7 +/- 0.1 nm. The higher angle part of the scattering profile, ...
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The disappearance of the guanosine triphosphate- (GTP) tubulin cap is widely believed to be the forerunner event for the growth-shrinkage transition ("catastrophe") in microtubule filaments in eukaryotic cells. We study a discrete version of a stochastic model of the GTP cap dynamics, originally proposed by Flyvbjerg, Holy, and Leibler [Phys. Rev. Lett. 73, 2372 (1994)]. Our model includes both...
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Microtubule-binding proteins (MBPs) are structurally and functionally diverse regulators of microtubule-mediated cellular processes. Alteration of MBPs has been implicated in the pathogenesis of human diseases, including cancer. MBPs can stabilize or destabilize microtubules or move along microtubules to transport various cargoes. In addition, MBPs can control microtubule dynamics through direc...
متن کاملMicrotubule protofilament number is modulated in a stepwise fashion by the charge density of an enveloping layer.
Microtubules are able to adjust their protofilament (PF) number and, as a consequence, their dynamics and function, to the assembly conditions and presence of cofactors. However, the principle behind such variations is poorly understood. Using synchrotron x-ray scattering and transmission electron microscopy, we studied how charged membranes, which under certain conditions can envelop pre-assem...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.50.33803