Characterization and Mass Spectrometry Analysis of Aminopeptidase N from Pseudomononas putida Lup.
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چکیده
منابع مشابه
FAST ATOM BOMBARDMENT MASS SPECTROMETRY (FABMS) ANALYSIS OF AN N- TERMINAL - BLOCKED PEPTIDE
FABMS analysis of T-lb peptide before and after one cycle of Edman degradation indicated an unblocked N-terminal Thr residue for this tryptic peptide. In contrast , our data showed a molecular protonated ion, MH + for T- la peptide at 655 mass units (mu) which is 42 mu higher than the MH ion of T- 1b peptide. In addition, T- la peptide was not amenable to one cycle of manual Edman degrada...
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An l-aminopeptidase of Pseudomonas putida, used in an industrial process for the hydrolysis of d,l-amino acid amide racemates, was purified to homogeneity. The highly l-enantioselective enzyme resembled thiol reagent-sensitive alkaline serine proteinases and was strongly activated by divalent cations. It possessed a high substrate specificity for dipeptides and alpha-H amino acid amides, e.g., ...
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Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-T...
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The purpose of this study was to isolate and characterize Proline Dehydrogenase (ProDH) enzyme frommicroorganisms isolated from soil in Iran. Isolation and screening of L-proline degradative enzymes from soilsamples was carried out. The isolate was characterized by biochemical markers and 16S rRNA geneanalysis. The target ProDH was purified and the effects of pH and temperatur...
متن کاملThe low-mass companion of GQ Lup
Using NACO on the VLT in the imaging mode we have detected an object at a distance of only 0.7 arcsec from GQ Lup. The object turns out to be co-moving. We have taken two K-band spectra with a resolution of λ/∆λ = 700. In here, we analyze the spectra in detail. We show that the shape of spectrum is not spoiled by differences in the Strehl ratio in the blue and in the red part, as well as differ...
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ژورنال
عنوان ژورنال: Polish Journal of Microbiology
سال: 2013
ISSN: 2544-4646,1733-1331
DOI: 10.33073/pjm-2013-046