Characterization of a Self-sufficient Trans-Anethole Oxygenase from Pseudomonas putida JYR-1
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چکیده
منابع مشابه
Characterization of a Self-sufficient Trans-Anethole Oxygenase from Pseudomonas putida JYR-1
A novel flavoprotein monooxygenase, trans-anethole oxygenase (TAO), from Pseudomonas putida JYR-1, which is capable of catalyzing the oxidation of trans-anethole to p-anisaldehyde, was heterologously expressed in E. coli and purified. Enzymatic kinetics of diverse substrates and cofactors revealed that TAO is likely to be a novel self-sufficient flavoprotein monooxygenase. Enzyme assays of GST-...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0073350