Characterization of Aberrant Melting Peaks in Unlabeled Probe Assays
نویسندگان
چکیده
منابع مشابه
SNP genotyping by unlabeled probe melting analysis.
Fluorescent nucleic acid detection in polymerase chain reaction (PCR) generally uses oligonucleotide probes labeled with covalently attached dyes. However, unlabeled oligonucleotides in the presence of saturating DNA dyes can also serve as hybridization probes. The DNA dye, LCGreen Plus, and a 3'-blocked unlabeled probe are added before amplification, and asymmetric PCR is performed at a 1:5 to...
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BACKGROUND JAK2 V617F, a somatic point mutation that leads to constitutive JAK2 phosphorylation and kinase activation, has been incorporated into the WHO classification and diagnostic criteria of myeloid neoplasms. Although various approaches such as restriction fragment length polymorphism, amplification refractory mutation system and real-time PCR have been developed for its detection, a gene...
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BACKGROUND The aim of our study was to establish an unlabeled-probe high-resolution melting (HRM) approach to the detection of Kirsten RAS (KRAS) codon 12 and 13 mutations in pancreatic adenocarcinoma (PA) tissues as a novel and effective diagnostic technique. METHODS We tested the sensitivity and specificity of this genotyping approach in cell lines with known KRAS mutations using 166 bp amp...
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Isolated-probe PCR (IP-PCR) is a method that combines asymmetric PCR, unlabeled probes, and high-resolution DNA melting while maintaining a closed tube system. A double-stranded DNA (dsDNA) dye LCGreen I was used to detect the unlabeled probes. LCGreen I is also used to detect the 277-base pair PCR product peak as an internal amplification control. To accomplish this, IP-PCR separates the asymm...
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We present the set of planar graphs that always have a simultaneous geometric embedding with a strictly monotonic path on the same set of n vertices, for any of the n! possible mappings. These graphs are equivalent to the set of unlabeled level planar (ULP) graphs that are level planar over all possible labelings. Our contributions are twofold. First, we provide linear time drawing algorithms f...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2007
ISSN: 1525-1578
DOI: 10.2353/jmoldx.2007.060139