Characterization of interleukin-1β mRNA expression in chicken macrophages in response to avian reovirus

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Characterization of interleukin-1beta mRNA expression in chicken macrophages in response to avian reovirus.

Inhibitors of viral disassembly or RNA and protein synthesis, viral disassembly intermediates (infectious subviral particles, ISVP), binary ethylenimine-inactivated virions, and viral particles lacking genomic double-stranded (ds) RNA (empty particles) were used to assess the expression of interleukin-1beta (IL-1beta) mRNA in chicken (chIL-1beta) macrophages in response to avian reovirus. The r...

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Evaluation of Immune Response to rσB Protein of Avian reovirus (ARV) in Chicken

Avian reovirus (ARV) belongs to the genus Orthoreovirus in the family Reoviridae and was first isolated from birds in 1954 (Fahey and Crawley, 1954). Virus particles are 70 nm to 80 nm in size, non-enveloped and have icosahedral symmetry with a double-shelled arrangement of surface protein. The genome of the virus is dsRNA with 10 segments. Depending on their electrophoretic mobility the genome...

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Abstract: iNOS is inducible by a variety of factors related to inflammation and referred to as inducible NOS(iNOS). It is regulated at the level of gene expression; once expressed, it produces NO at a high rate. iNOS gene-expression profiling is an important tool in understanding molecular markers of the responses of cells and tissues to external factors. In this article a semiquantitative reve...

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Inoculation of avian oncoviruses into 1- to 2-month old chickens led to a rapid production of antiviral humoral antibodies. Under these conditions it was found that avian leukosis viruses are sequestered in macrophages of peripheral blood, in which they can persist for a long period of time (up to about 3 years). In contrast, avian sarcoma viruses were never found in macrophages from chickens d...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 2008

ISSN: 0022-1317,1465-2099

DOI: 10.1099/vir.0.82957-0