Characterization of Luminal Paneth Cell α-Defensins in Mouse Small Intestine
نویسندگان
چکیده
منابع مشابه
Paneth Cell Granule of Mouse Intestine
The Paneth cell secretion of the mouse intestine has been reported to be an acid mucopolysaccharide-protein complex; tryptophan as well as alpha acylamido carboxyl, amino, and phenolic groups of protein have been localized to the characteristic granules by means of histochemical staining procedures (13). Sulfhydryl and disulfide groups of protein have also been localized to the granule (14). Th...
متن کاملDifferential Susceptibility of Bacteria to Mouse Paneth Cell α-Defensins under Anaerobic Conditions
Small intestinal Paneth cells secrete α-defensin peptides, termed cryptdins (Crps) in mice, into the intestinal lumen, where they confer immunity to oral infections and define the composition of the ileal microbiota. In these studies, facultative bacteria maintained under aerobic or anaerobic conditions displayed differential sensitivities to mouse α-defensins under in vitro assay conditions. R...
متن کاملA monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for detection of secreted α-defensin.
Paneth cells at the base of small intestinal crypts secrete α-defensins, which contribute to innate immunity and shape composition of enteric microbiota. Efforts to establish a relationship between secreted α-defensins and disease have been hampered by a lack of sensitive assays to quantify luminal α-defensins. Here we report on a highly sensitive sandwich enzyme-linked immunosorbent assay (ELI...
متن کاملPaneth cell α-defensins and enteric microbiota in health and disease
Antimicrobial peptides are major effectors of innate immunity of multicellular organisms including humans and play a critical role in host defense, and their importance is widely recognized. The epithelium of the intestine is the largest surface area exposed to the outer environment, including pathogens, toxins and foods. The Paneth cell lineage of intestinal epithelial cells produces and secre...
متن کاملStrain-specific polymorphisms in Paneth cell α-defensins of C57BL/6 mice and evidence of vestigial myeloid α-defensin pseudogenes.
Paneth cells at the base of small intestinal crypts secrete microbicidal α-defensins, termed cryptdins (Crps) in mice, as mediators of innate immunity. Proteomic studies show that five abundant Paneth cell α-defensins in C57BL/6 mice are strain specific in that they have not been identified in other inbred strains of mice. Two C57BL/6-specific peptides are coded for by the Defcr20 and -21 genes...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.m004062200