Characterization of the integration protein of bacteriophage lambda as a site-specific DNA-binding protein.
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چکیده
منابع مشابه
Characterization of the integration protein of bacteriophage lambda as a site-specific DNA-binding protein.
The Int protein specified by bacteriophage lambda is required for the recombination event that integrates the viral DNA into the host genome at its specific attachment site. Using a DNA-binding assay, we have partially purified the Int protein and studied some of the features of its binding specificity and regulation. The DNA-binding activity is attributed to Int protein because the activity is...
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Quantitative analysis of nitrocellulose filter binding data by the method of Clore, Gronenborn and Davies [(1982) J. Mol. Biol. 155, 447-466] has been used to show that lambda integration protein (Int) exhibits cooperativity in binding to specific recognition sites within the attachment site region (lambda attP) of bacteriophage lambda DNA. Optimal values of the equilibrium constant obtained we...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1977
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.74.4.1511