CHARACTERIZATION OF TWO HELA SUBLINES: TCRC-1 PRODUCES REGAN ISOENZYME AND TCRC-2, NON-REGAN ISOENZYME

Characterization of Two Hela Sublines: Tcrc-1 Produces Regan Isoenzyme and Tcrc-2, Non-regan Isoenzyme

HeLa Cells have been found to produce a placental form of alkaline phosphatase (1, 8, 17) which is very similar to the Regan isoenzyme, a carcinoplacental antigen found in cancer patients (7-9) . In order to investigate the nature of gene expression of carcinoplacental proteins, HeLa cells were cloned, and a subline (HeLa TCRC-1), monophenotypic for the Regan isoenzyme was selected . The proper...

Regan isoenzyme: a carcinoplacental antigen.

Phenotypes of the Regan isoenzyme and identity between the placental D-variant and the Nagao isoenzyme.

We observed an unexpectedly high incidence of the D-variant placenta! phenotype in Regan isoenzyme-positive sera of cancer patients-. Such D-variant-positive sera exhibit a high degree of inhibition by L-leucine, a phenomenon earlier associated with the Nagao isoenzyme. Unlike normal pla centae, a diffuse phenotype of heat-stable alkaline phosphatase is usually noted in cancer sera containing N...

Elevation of histaminase and its concurrence with Regan isoenzyme in ovarian cancer.

Histaminase has been shown to be associated with several types of human cancer. In the present study, we examined the activity of histaminase and its relationship with Regan isoenzyme of alkaline phosphatase in ascitic fluids obtained from patients with ovarian and several other types of cancer. We have found that about 44% of the ovarian cancer patients had elevated levels of histaminase in th...

Evidence for altered gene regulation in HeLa cells returned to culture after growing in immunosuppressed rats.

HeLa TCRC-1, is a monophenotypic cell line for the production of the carcinoplacental Regan isoenzyme of alkaline phosphatase. It has been previously reported that, when this cell line is grown in immunosuppressed rats, there is an alteration of phenotypic expression in that an isoenzyme that is referred to as oncoamnion (FL) becomes the dominant enzyme form. This report describes the isoenzyme...