CHEMICAL SELECTION OF ALCOHOL DEHYDROGENASE NEGATIVE MUTANTS IN DROSOPHILA
نویسندگان
چکیده
منابع مشابه
Chemical selection of alcohol dehydrogenase negative mutants in drosophila.
We describe a selection procedure which utilizes the vapor from an unsaturated alcohol, 1-pentene-3-ol, for the detection and isolation of mutant flies with little or no alcohol dehydrogenase activity. ADH-negative flies are unaffected by exposure to the unsaturated alcohol, but ADH positives (wild-types) die after short exposure. The technique can be used to select rare ADH-negative individual...
متن کاملChemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol.
We describe a procedure for the selection of alcohol dehyrogenase negative mutants in Drosophila. The method consists of exposing eggs and larvae to low concentrations of 1-pentyne-3-ol dissolved in the culture medium. Only those flies with greatly reduced levels of alcohol dehydrogenase activity survive. In addition, genotypically negative flies die if their mothers are alcohol dehydrogenase p...
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Sixteen Adh-negative mutants (induced by ethyl methanesulfonate) were examined for the presence of inactive alcohol dehydrogenase (ADH) protein. Four techniques were utilized in an effort to detect this protein: hybrid enzyme formation, intra-cistronic complentation, sodium dodecyl sulfate electrophoresis and antibody precipitation. Eleven of the sixteen negative strains showed evidence of inac...
متن کاملA pollen selection system for alcohol-dehydrogenase-negative mutants in plants.
Exposure of freshly shed maize pollen to allyl alcohol vapors allows selective fertilization by mutant alcohol-dehydrogenase-negative grains. Wild-type pollen grains are killed by the enzymatic conversion of allyl alcohol to the highly toxic acrylaldehyde.
متن کاملIsolation of trans-acting regulatory mutations in Drosophila using alcohol dehydrogenase gene fusions for chemical selection
Alcohol Dehydrogenease (Adh) is an ideal enzyme for chemical selection of mutations affecting its expression, as Adh activity is required for survival of exposure to ethanol. Conversely, Adh activity renders exposure to 3-pentyne-l-ol lethal, thus allowing the selection of Adh-deficient flies. We have sought to adapt this system to the isolation of mutations affecting the expression of developm...
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ژورنال
عنوان ژورنال: Genetics
سال: 1972
ISSN: 1943-2631
DOI: 10.1093/genetics/72.3.545