Chicken Feathers Waste Management by Microbial as a Sustainable and Tool Environmental Friendly

Valorizing chicken feather agro-wastes nowadays is imperative, if these wastes are disposed of without treatment, they could contribute to environmental problems. Conventional methods processing feathers such as landfilling, chemical treatment and burning costly, non-time consumable toxic the environment. Microbial hydrolysis, on contrary, now considered main environmental-friendly recycling alternative. Hence, aim this study isolate keratinolytic bacteria with efficient optimize some physical parameters that affect both bacterial growth consequently its degrading ability. Results demonstrated an ability newly identified Bacillus sp. D4 strain isolated from under optimal mesophilic temperature 37°C, pH 8.0 106 CFU/mL cell size inoculum, interpreted by highest keratin activity (55.0 ± 1.35 U/mL) (54.3 1.5 respectively higher total protein content in free supernatant 0.65 mg/mL. Beyond values, a moderate enzyme was observed at 40°C (35.1 2 U/mL), 25°C (30.1 similarly initial 7.5 (52.3 9.0 (49.0 1.2 10.0 (38.2 U/mL). D4, other hand, not able tolerate high alkaline value 11.0 nor acidic 4.0 5.0 55°C, correspondingly low noted (19.0 1