Chicken lens δ-crystallin gene expression and methylation in several non-lens tissues
نویسندگان
چکیده
منابع مشابه
Cooperative action between L-Maf and Sox2 on δ-crystallin gene expression during chick lens development
Lens development is regulated by a variety of transcription factors with distinct properties. The lens-specific transcription factor, L-Maf, is essential for lens formation and induces lens-specific markers, such as the crystallin genes. In this study, we analyzed the mechanism by which L-Maf regulates delta-crystallin expression. Misexpression of L-Maf in the head ectoderm of lens placode-form...
متن کاملExpression in non-lens tissues of an enzyme activity related to the 'lens-specific' protein, delta crystallin.
When chick embryo neutral retina (NR) cells are cultured for long periods in vitro, they undergo extensive transdifferentiation into lens and express the lens protein, delta crystallin. We now demonstrate that this process is accompanied by a change in the chromatin conformation of the delta-gene locus from DNAase1-resistant to DNAase1-sensitive in the nuclei of most cells. Transcripts hybridis...
متن کاملCloning and Mapping the Mouse Crygs Gene and Non-lens Expression of γS-Crystallin
and patterns of expression, γ-crystallins have generally appeared to be the most lens-specific of the crystallins. Here we examine the mouse γS-crystallin (γS) gene and its expression. Methods: The cDNA and gene for mouse γS were cloned and sequenced. The Crygs gene was mapped using genetic crosses. Expression patterns in mouse and cow were examined by northern blot, PCR and western blot using ...
متن کاملAnalysis of non-crystallin lens fiber cell gene expression in c-Maf -/- mice.
PURPOSE To examine the role of the c-Maf transcription factor in the regulation of non-crystallin lens fiber cell specific gene expression. METHODS Expression of lens fiber cell genes in c-Maf null mice was analyzed by immunohistochemical and RT-PCR methods. In addition, the effect of c-Maf on fiber cell gene promoter fragments was examined in co-transfection experiments. RESULTS Examinatio...
متن کاملeye-lens protein y-crystallin
Although ubiquitous, no universal function can be confidently ascribed to glutathione. The eye lens maintains higher concentrations of reduced glutathione within its cells than do other tissues (Waley, 1969). The ratio of protein thiol to reduced glutathione in most species of lens is about 5:1 (Kuck et al., 1982). The requirement for glutathione in maintaining a transparent lens is indicated b...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1983
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/11.9.2513