Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences

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Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences1

PREMISE OF THE STUDY Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome ass...

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Chloroplast microsatellite markers for Pseudotaxus chienii developed from the whole chloroplast genome of Taxus chinensis var. mairei (Taxaceae)1

PREMISE OF THE STUDY Pseudotaxus chienii (Taxaceae) is an old rare species endemic to China that has adapted well to ecological heterogeneity with high genetic diversity in its nuclear genome. However, the genetic variation in its chloroplast genome is unknown. METHODS AND RESULTS Eighteen chloroplast microsatellite markers (cpSSRs) were developed from the whole chloroplast genome of Taxus ch...

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Characterization of microsatellite markers for Broussonetia papyrifera (Moraceae)1

PREMISE OF THE STUDY Broussonetia papyrifera (Moraceae) is native to Asia and is used as a medicinal plant and as a source of fiber for making paper. It was dispersed into the Pacific region as a fiber source for making nonwoven textiles (barkcloth). Microsatellites were developed to trace the human-mediated dispersal of this species into the Pacific region. METHODS AND RESULTS A set of 36 mi...

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Development of microsatellite loci in Artocarpus altilis (Moraceae) and cross-amplification in congeneric species1

UNLABELLED PREMISE OF THE STUDY Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit) and tested in four Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus. • METHODS AND RESULTS A total of 25 microsatellite loci were evaluated across four Artocarpus species and o...

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Development and characterization of EST-SSR markers for Artocarpus hypargyreus (Moraceae)1

PREMISE OF THE STUDY Polymorphic microsatellite markers were developed for Artocarpus hypargyreus (Moraceae), a threatened species endemic to China, to investigate the genetic diversity and structure of the species. METHODS AND RESULTS Based on the transcriptome data of A. hypargyreus, 63 primer pairs were preliminarily designed and tested, of which 34 were successfully amplified and 10 displ...

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ژورنال

عنوان ژورنال: Applications in Plant Sciences

سال: 2015

ISSN: 2168-0450,2168-0450

DOI: 10.3732/apps.1500049