Chloroplast rpoA, rpoB, and rpoC genes specify at least three components of a chloroplast DNA-dependent RNA polymerase active in tRNA and mRNA transcription.
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چکیده
منابع مشابه
Preferential transcription of cloned maize chloroplast DNA sequences by maize chloroplast RNA polymerase.
Zea mays chloroplast DNA-dependent RNA polymerase in vitro preferentially transcribes maize chloroplast DNA sequences incorporated in cloned chimeric bacterial plasmids. Preferential transcription is dependent on the presence of a 27.5-kilodalton polypeptide, the S factor, which has been purified from maize chloroplasts, and also on the template's being in the supercoiled form.
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Prominent polypeptides with apparent molecular masses of 180, 120, 85, and 38 kDa are found in an extensively purified preparation of maize chloroplast DNA-dependent RNA polymerase that retains the capacity to initiate transcription of the cloned chloroplast gene rbcL correctly and the requirement for a supercoiled DNA template for specific and active transcription. Amino-terminal amino acid se...
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Plastid genes are transcribed by DNA-dependent RNA polymerase(s), which have been incompletely characterized and have been examined in a limited number of species. Plastid genomes contain rpoA, rpol, rpoC1, and rpoC2 coding for a, 8, B', and 8" RNA polymerase subunits that are homologous to the a, 8, and 8' subunits that constitute the core moiety of RNA polymerase in bacteria. However, genes w...
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To determine whether chloroplast RNA polymerase will accurately terminate transcription in vitro, we have fused the spinach chloroplast rbcL promoter to the 3' end of the rbcL gene as well as to various factor independent transcription terminators from E. coli. Transcription of the rbcL minigene did not result in production of the expected 265 nucleotide RNA. However, the spinach chloroplast RN...
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Clones including tRNA genes from tomato {Lycopersicon esculentum) chloroplast DNA have been obtained. Here we report the nucleotide sequence of tmH\ trnD-^ and tmL\ Sequences were determined by the dideoxy method (1). In higher plant chloroplast genomes, tmY and trnD are clustered together with trnE in the large single copy (LSQ region (2). The sequence of trnY reveals 100% identity with its co...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)68221-3