Chromatin tandem affinity purification sequencing
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چکیده
منابع مشابه
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With the number of complete genome sequences increasing at an ever-faster pace, we are getting sequence data for the full complement of proteins or “proteome” (Wasinger et al. 1995; Wilkins et al. 1996), encoded by various organisms. To understand how the proteome confers specific properties to a host cell, one needs to obtain information regarding several parameters, including the level of exp...
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TAP (tandem affinity purification) allows rapid and clean isolation of a tagged protein along with its interacting partners from cell lysates. Initially developed in yeast, the TAP method has subsequently been adapted to other cells and organisms. In combination with MS analysis, this method has become an indispensable tool for systematic identification of target-associated protein complexes. T...
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A critical and often limiting step in understanding the function of host and viral proteins is the identification of interacting cellular or viral protein partners. There are many approaches that allow the identification of interacting partners, including the yeast two hybrid system, as well as pull down assays using recombinant proteins and immunoprecipitation of endogenous proteins followed b...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2013
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2013.088