Chromatographic resolution of complementary strands of denatured Pneumococcal DNA.
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چکیده
منابع مشابه
Chromatographic resolution of complementary strands of denatured Pneumococcal DNA.
It has been shown that AMAK columns are capable of resolving the complementary strands of pneumococcal DNA. Using stepwise elution of denatured DNA, two fractions were obtained at different eluting salt molarities, which regained high biological activity on annealing only when previously mixed.' Unfortunately, resolution could not clearly be demonstrated by means of chromatographic profiles the...
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A Fortran computer algorithm has been used to analyze the nucleotide sequence of several structural genes. The analysis performed on both coding and complementary DNA strands shows that whereas open reading frames shorter than 100 codons are randomly distributed on both DNA strands, open reading frames longer than 100 codons ("virtual genes") are significantly more frequent on the complementary...
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The buoyant density of DNA in the CsCl equilibrium density gradient depends on its composition and secondary structure. Denatured DNA's usually form a single band (Fig. 1B and F) at a density 0.013 (high % G + C) to 0.017 (low % G + C) gm/cm3 higher than that of the same DNA in its native helical form (Fig. 1A and E). Experience with Escherichia coli (Fig. iF and H) and several other organisms ...
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MOTIVATION Over sufficiently long windows, complementary strands of DNA tend to have the same base composition. A few reports have indicated that this first-order parity rule extends at higher orders to oligonucleotide composition, at least in some organisms or taxa. However, the scientific literature falls short of providing a comprehensive study of reverse-complement symmetry at multiple orde...
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We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage lambda DNA. The 3' and 5' extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10-15 pN. The separation force signal is shown to be related to the local GC vs. AT...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1968
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.59.1.200