Chromogenic in situ detection of microRNAs in paraffin embedded tissue microarrays by locked nucleic acid probes
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چکیده
منابع مشابه
Identification of Fusarium species in formalin-fixed and paraffin-embedded sections by in situ hybridization using peptide nucleic acid probes.
Fusarium has recently emerged as an opportunistic pathogen of humans, but the histological differentiation of Fusarium from Aspergillus and Scedosporium is particularly difficult because these fungi may induce similar clinical features and exhibit filamentous development in host tissues. Thus, there is a need to establish rapid and reliable methods that are applicable to pathological diagnoses....
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Double-stranded probes are homogeneous biosensors for rapid detection of specific nucleotide sequences. These double-stranded probes have been applied in various molecular sensing applications, such as real-time polymerase chain reaction and detection of bacterial 16S rRNA. In this study, we present the design and optimization of double-stranded probes for single-cell gene expression analysis i...
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Human papillomaviruses (HPVs) infect mucosal and cutaneous epithelia and cause malignant and benign neoplastic lesions. Human papillomaviruses cause 250000 deaths per year from cervical cancer most often in developing countries. This major public health problem make them important targets in the search for Human papillomaviruses detection methods that may play a role in cervical cancer screen...
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BACKGROUND Although nucleic acid derangements are the hallmark of melanocytic dysplasia, the gold standard for its diagnosis remains the microscopic evaluation of haematoxylin and eosin stained slides. However, light microscopy is subjective and crucial genomic changes do not always show as changes in histology. AIMS To introduce the nucleic acid index (NAI) as a means of analysing nucleic ac...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2012
ISSN: 2043-0116
DOI: 10.1038/protex.2012.030