Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis dormancy-associated antigens Rv2659c and Rv3128c in active and latent tuberculosis

ObjectiveTo elucidate the antigenic potential of dormancy-associated antigens Rv2659c and Rv3128c Mycobacterium tuberculosis by examining persistence specific IgG IgA memory B cells (MBCs) among patients with active (TB), household contacts latent (LTBI), an endemic healthy control group.MethodsFresh peripheral blood mononuclear from three study groups were used to enumerate numbers MBCs recombinant protein ELISpot assay. The composition MBC subsets IgA+ + was analyzed flow cytometry.ResultsThe number antigen significantly higher in LTBI group than TB group. In contrast, no significant difference found or against Rv3128c. that IgG+ classical subset group.ConclusionThe results indicated induced response individuals TB, formed a major portion subset. This new knowledge will be beneficial for development novel vaccines their TB.