Clinical quantitative flow cytometry: ?Identifying the optimal methods for clinical quantitative flow cytometry?
نویسندگان
چکیده
منابع مشابه
Flow cytometry in the clinical laboratory.
This issue of the eJIFCC is dedicated to highlight some aspects of clinical flow cytometry. In the past 50 years several techniques have revolutionized laboratory medicine. Undoubtedly flow cytometry is one of those, with a substantial impact on diagnosing and monitoring diseases in laboratory hematology, hemostasis and immunology. The development in flow technology also exerted a considerable ...
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We report here a quick and inexpensive method for preparing standards of known fluorochrome content for calibration and quantitation of flow cytometry fluorescence signals. Erythrocyte ghosts prepared by hypotonic lysis are filled with solutions containing fluorescently labeled dextran. Standards prepared by this technique have a narrow range of fluorescence and a linear response of fluorescenc...
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Immunophenotyping by flow cytometry is a robust and highly complex technology used in the enumeration and characterization of leukocytes, including normal lymphocyte subsets and hematologic neoplasms. Samples consist of peripheral blood and many times irreplaceable samples, such as bone marrow and fresh tissue. These samples are collected, transported, prepared, analyzed, and interpreted, resul...
متن کاملApplications of Flow Cytometry to Clinical Microbiology
Microbiology in general and clinical microbiology in particular have witnessed important changes during the last few years. Traditional methods of bacteriology and mycology require the isolation of the organism prior to identification and other possible testing. In most cases, culture results are available in 48 to 72 h. Virus isolation in cell cultures and detection of specific antibodies have...
متن کاملApplications of flow cytometry to clinical microbiology.
Classical microbiology techniques are relatively slow in comparison to other analytical techniques, in many cases due to the need to culture the microorganisms. Furthermore, classical approaches are difficult with unculturable microorganisms. More recently, the emergence of molecular biology techniques, particularly those on antibodies and nucleic acid probes combined with amplification techniq...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2003
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.b.10053