Cloning and expression of a beta tubulin gene of Physarum polycephalum
نویسندگان
چکیده
منابع مشابه
The localization of the divergent beta 2-tubulin isotype in the microtubular arrays of Physarum polycephalum.
The beta 2-tubulin isotype of Physarum polycephalum is only 83% identical in amino acid sequence with the constitutively expressed beta 1B-tubulin and the myxamoeba-specific beta 1A-tubulin isotypes. A polyclonal antibody specific for beta 2-tubulin was used to monitor the subcellular distribution of the beta 2-tubulin antigen in the mitotic spindle of the mature plasmodium - the sole microtubu...
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The expression and cytological distribution of acetylated alpha-tubulin was investigated in Physarum polycephalum. A monoclonal antibody specific for acetylated alpha-tubulin, 6-11B-1 (Piperno, G., and M. T. Fuller, 1985, J. Cell Biol., 101:2085-2094), was used to screen for this protein during three different stages of the Physarum life cycle--the amoeba, the flagellate, and the plasmodium. We...
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Coronin cDNA was cloned from the plasmodia of Physarum polycephalum. The amino acid sequence deduced from the cDNA was comprised of 449 residues and showed 60% identity to that of Dictyostelium discoideum coronin. Southern blot analysis suggested that the coronin gene present in the P. polycephalum genome might be a single copy. Coronin was expressed in diploid plasmodia, while it was not detec...
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In the paper we proposed a novel model of unconventional computing where a structural part of computation is presented by dynamics of Plasmodium of Physarum polycephalum, a large single cell. We sketch a new logical approach combining conventional logic with process calculus to demonstrate how to employ formal methods in design of unconventional computing media presented by Physarum polycephalu...
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Physarumpolycephalum is an acellular slime mould whose histones are of general interest because they allow comparisons to be made between lower eukaryote and higher eukaryote histones and, perhaps more importantly, they provide a unique opportunity for the study of histone changes in the mitotic cycle, which is naturally synchronous in P. polycephalum plasmodia, and during differentiation. Stud...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1988
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1988.tb14125.x