Cloning, Expression and Purification of Recombinant Interleukin 1 Receptor Antagonist (IL-1RA) in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Evaluation of the expression of interleukin 1 beta (IL-1beta) and interleukin 1 receptor antagonist (IL-1Ra) in leprosy patients.
Leprosy is an infectious and contagious spectral disease accompanied by a series of immunological events triggered by the host's response to the etiologic agent, Mycobacterium leprae. Evidence suggests that the induction and maintenance of the immune/inflammatory response in leprosy are linked to multiple cell interactions and soluble factors, mainly through the action of cytokines. The ELISA t...
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Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...
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ژورنال
عنوان ژورنال: SSR Institute of International Journal of Life Sciences
سال: 2020
ISSN: 2581-8732,2581-8740
DOI: 10.21276/ssr-iijls.2020.6.3.1