Cloning of apoB intrabodies: Specific knockdown of apoB in HepG2 cells
نویسندگان
چکیده
منابع مشابه
Effects of g-Tocotrienol on ApoB Synthesis, Degradation, and Secretion in HepG2 Cells
g-Tocotrienol (g-T3), a naturally occurring analog of tocopherol (vitamin E), has been shown to have a hypocholesterolemic effect in animals and humans. Unlike tocopherol, it has also been shown to reduce plasma apoB levels in hypercholesterolemic subjects. The aim of this study was to define the mechanism of action of g-T3 on hepatic modulation of apoB production using cultured HepG2 cells as ...
متن کاملEffects of gamma-tocotrienol on ApoB synthesis, degradation, and secretion in HepG2 cells.
gamma-Tocotrienol (gamma-T3), a naturally occurring analog of tocopherol (vitamin E), has been shown to have a hypocholesterolemic effect in animals and humans. Unlike tocopherol, it has also been shown to reduce plasma apoB levels in hypercholesterolemic subjects. The aim of this study was to define the mechanism of action of gamma-T3 on hepatic modulation of apoB production using cultured Hep...
متن کاملSpecific in vivo knockdown of protein function by intrabodies
Intracellular antibodies (intrabodies) are recombinant antibody fragments that bind to target proteins expressed inside of the same living cell producing the antibodies. The molecules are commonly used to study the function of the target proteins (i.e., their antigens). The intrabody technology is an attractive alternative to the generation of gene-targeted knockout animals, and complements kno...
متن کاملNiacin accelerates intracellular ApoB degradation by inhibiting triacylglycerol synthesis in human hepatoblastoma (HepG2) cells.
The mechanism by which the potent drug niacin decreases apoB-containing atherogenic lipoproteins and prevents coronary disease is unclear. Utilizing human hepatoblastoma (HepG2) cells as an in vitro model, we have examined the effect of niacin on intracellular degradation of apoB and the regulatory mechanisms involved in apoB processing. Niacin significantly increased apoB degradation in a dose...
متن کاملPostprandial changes of apoB-100 and apoB-48 in TG rich lipoproteins in familial combined hyperlipidemia.
Impaired chylomicron (chylo) remnant clearance and small VLDL overproduction are major metabolic abnormalities in familial combined hyperlipidemia (FCHL). Quantitative data on postprandial apolipoprotein B-48 (apoB-48) and apolipoprotein B-100 (apoB-100) in TG rich lipoproteins (TRL) in FCHL have not been reported before. Eight untreated FCHL patients and 10 matched controls underwent a 24 h or...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 2008
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2008.06.020