Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells
نویسندگان
چکیده
منابع مشابه
Monoclonal Antibodies
SUMMARY Monoclonal Antibodies are identical antibodies and Can be generated in ultimated amounts by countinuos cultures of single antibody- secreting cells. These cell lines are produced by cell fusion of normal lymphocytes to cells of a myeloma tumor cell line, which confers on the antibody producing hybrid cell, immortality and the ability grow as atumor in animals. monoclonal antibodies c...
متن کاملEngineering Hydrophobic Protein–Carbohydrate Interactions to Fine-Tune Monoclonal Antibodies
Biologically active conformations of the IgG1 Fc homodimer are maintained by multiple hydrophobic interactions between the protein surface and the N-glycan. The Fc glycan modulates biological effector functions, including antibody-dependent cellular cytotoxicity (ADCC) which is mediated in part through the activatory Fc receptor, FcγRIIIA. Consistent with previous reports, we found that site-di...
متن کاملImmunization with SV40-transformed cells yields mainly MHC-restricted monoclonal antibodies
Recognition of antigens on cell surfaces only in the context of the MHC-encoded alloantigens of the presenting cell (self + X) has classically been considered the province of T cells. However, evidence from several sources has indicated that B cells and antibodies can exhibit self + X-restricted recognition as well. This report concerns the mAb response to SV40-transformed H-2b fibroblast cell ...
متن کاملMonoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design
Establishing stable Chinese Hamster Ovary (CHO) cells producing monoclonal antibodies (mAbs) usually pass through the random integration of vectors to the cell genome, which is sensitive to gene silencing. One approach to overcome this issue is to target a highly transcribed region in the genome. Transposons are useful devices to target active parts of genomes, and PiggyBac (PB) transposon can ...
متن کاملReduction in C-terminal amidated species of recombinant monoclonal antibodies by genetic modification of CHO cells
BACKGROUND During development of recombinant monoclonal antibodies in Chinese hamster ovary (CHO) cells, C-terminal amidated species are observed. C-terminal amidation is catalysed by peptidylglycine α-amidating monooxygenase (PAM), an enzyme known to be expressed in CHO cells. The significant variations between clones during clone selection, and the relatively high content of amidated species ...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2017
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.26375