Compact type-I coil planet centrifuge for counter-current chromatography
نویسندگان
چکیده
منابع مشابه
Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge.
This review describes protein purifications from cell culture medium or cell-lysate by high speed counter-current chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.
متن کاملPurification of Sesquiterpenes from Saussurea LappaRoots by High Speed Counter Current Chromatography
Sesquiterpenes lactones including costunolide and dehydrocostus lactone, were isolated from Saussurea lappa roots, which had exhibited a wide range of biological activities such as anti-cancer, anti-inflammatory, antiulcer, and immunomodulatory activities. High-speed counter-current chromatography (HSCCC) was applied for the rapid preparative isolation of sesquiterpenes lactones from <...
متن کاملPurification of Sesquiterpenes from Saussurea LappaRoots by High Speed Counter Current Chromatography
Sesquiterpenes lactones including costunolide and dehydrocostus lactone, were isolated from Saussurea lappa roots, which had exhibited a wide range of biological activities such as anti-cancer, anti-inflammatory, antiulcer, and immunomodulatory activities. High-speed counter-current chromatography (HSCCC) was applied for the rapid preparative isolation of sesquiterpenes lactones from <...
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We developed a n-hexane/surfactant-containing water solvent system in counter-current chromatography (CCC) in order to separate hydrophobic compounds. By using the upper phase as the mobile phase, we have separated steroid samples. Retention times of steroids progesterone and delta4-androstene-3,17-dione increased slightly by increasing the concentration below the critical micellar concentratio...
متن کاملSample capacity in preparative high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) is a versatile technique in preparative separation and purification of pure compounds from complex matrices. As a preparative chromatography, there is a need to maximize the column production. Based on the plate theory of Van Deemter, the effect of the sample load on the separation was investigated in a preparative HSCCC with a 1000 ml column ca...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2010
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2009.12.055