Comparative Assessment of Various Concentration and Exposure Time of Sodium Dodecyl Sulfate as Decellularization Agents for Small-Vessels Vascular Tissue Engineering

BACKGROUND: Finding the optimum vascular grafts (VG) to replace damaged blood arteries in cardiac surgery is still a work progress. To be employed, tissue-engineered VG (TEVG) must have appropriate biological and mechanical qualities. Decellularized may better TEVG than synthetic because of their natural three-dimensional architecture. AIM: The goal this study was compare different concentrations times sodium dodecyl sulfate (SDS) decellularize tissue find best decellularized VG. METHODS: In all scaffolds, which are 1% SDS-2 weeks group, hematoxylin eosin Masson’s trichrome staining exhibited looser collagen networks fewer nuclei. RESULTS: orientation fibers identical native scaffolds. Collagen I deposition seen immunohistochemistry assay. A tensile strength test revealed that scaffold (0.5% SDS for 4 0.5% 2 weeks) had exceeded arteries’ maximal strength. comparison treated groups, scanning electron microscopy following decellularization no endothelial cells on inner side group with minimum extracellular matrix damage. remained marginally visible groups. 3-(4,5-dimethylthiazol-2yl)2,5-diphenyltetrazolium bromide used determine cytotoxicity CONCLUSION: This reveals exposing bovine mesenteric artery an excellent procedure extracting most acellular VG, potentially serving as TEVGs.