Comparative Glycomic Analysis of Sialyl Linkage Isomers by Sialic Acid Linkage-Specific Alkylamidation in Combination with Stable Isotope Labeling of α2,3-Linked Sialic Acid Residues
نویسندگان
چکیده
منابع مشابه
Sialic Acid Fluorescence Labeling Kit
I. Description ............................................................................................2 II. Kit Components .................................................................................2 III.Storage ..................................................................................................2 VI.Procedure.................................................................
متن کاملThe role of sialic acid synthases in sialic acid biosynthesis
Introduction The sialic acids comprise a diverse family of nine-carbon keto-sugars, the majority of which are structural derivatives of neuraminic acid (Fig. 1). Neuraminic acid has an amino moiety at the C-5 position, and carboxylate at C-2 which confers negative charge at physiological pH. Whereas unsubstituted neuraminic acid is not known to exist in nature, over 40 substituted variations (c...
متن کاملAssessment of Sialic Acid Distribution in Mouse Epididymis
Previous studies have shown that epididymal epithelium and its secretions are critical for sperm maturation. These secretions contain many glycoconjugates with sialic acid terminal sugar. This terminal sugar by interveining in cellular interactions and masking surface receptors, has an important role in sperm maturation and protection. Moreover lectins have been employed as useful probes to det...
متن کاملImproved method for fluorescence labeling of sugar chains with sialic acid residues.
The pyridylamination method is useful for the structural unalysis ef sugar chains from smal] arnounts of glycoproteins. About 60(;・g of the sialyl linkage was, however, hydrolyzed under the reaction conditions reported previously.i2) The grewing need for fluorescence labeling of sialyl sugar chains prompted us to re-investigate the reaction conditions. Recently. wc tried pyridylamination condit...
متن کاملSurface labeling of sialic acid residues in BHK cells and viral transformants.
Conditions were found for the direct radioactive labeling of glycoproteins on the membranes of baby hamster kidney fibroblasts and their Rous sarcoma virus transformants with negli gible loss of viability. Sialic acid residues were specifically detected by oxidation of the cells with periodate followed by reduction with sodium borotritide. Highly specific incorporation of radioactivity was demo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2019
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.9b03617