Comparative Study of Various Procedures for Extracting Doxorubicin from Animal Tissue Samples

This article presents a comparative study of selected deproteinization-, liquid–liquid-extraction- (LLE), and solid-phase-extraction (SPE)-based procedures for the isolation doxorubicin (DOX) daunorubicin (DAU) as an internal standard (IS) from rat tissue samples. During experiments, all samples were analyzed via liquid chromatography coupled with fluorescence detection (LC-FL), analytes being monitored at excitation emission wavelengths 487 555 nm, respectively. The absolute recoveries sample-preparation procedure then calculated compared, advantages disadvantages each approach considered in depth. Ultimately, SPE hydrophilic–lipophilic balanced (HLB) sorbents was most effective extraction it enabled recovery DOX level 91.6 ± 5.1%. Next, HLB-SPE protocol LC-FL separation resultant method validated according to FDA ICH requirements. validation data confirmed that developed met required criteria bioanalytical methods, limit (LOD) quantification (LOQ) 0.005 µg/g 0.01 µg/g, Finally, successfully tested on various tissues enriched DOX, confirming its potential interesting alternative previously reported protocols pharmacokinetic studies clinical investigations aimed analysis biodistribution after systemic administration this drug.