Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Hemolymph in Response to Autographa californica Nucleopolyhedrovirus in Differentially Resistant Strains

Bombyx mori nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although Autographa californica (AcMNPV) and BmNPV are both baculoviruses, the host domains these two viruses have almost no intersection in nature. Recently, it has been found some silkworms could be infected by recombinant AcMNPV through puncture, which provided valuable material for studying infection mechanism baculovirus silkworm. In this study, comparative transcriptomics was used analyse hemolymph differentially resistant strains following inoculation. There were 678 DEGs p50 515 C108 viral infection. Among them, upregulation downregulation similar p50; however, upregulated nearly twice as numerous downregulated C108. The different differed GO enrichment. Based on KEGG enrichment, mainly enriched metabolic pathways apoptosis pathway Moreover, 13 genes involved 11 analysed. apoptosis, function BmTex261 showed highest expression significant response C108, indicating anti-AcMNPV This further validated significantly decreased gene lef3 after overexpression BmN cells. results provide theoretical reference molecular resistance silkworms.