Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro
نویسندگان
چکیده
منابع مشابه
The in vitro regulation of ovarian follicle development using alginate-extracellular matrix gels.
The extracellular matrix (ECM) provides a three-dimensional structure that promotes and regulates cell adhesion and provides signals that direct the cellular processes leading to tissue development. In this report, synthetic matrices that present defined ECM components were employed to investigate these signaling effects on tissue formation using ovarian follicle maturation as a model system. I...
متن کاملThe role of the extracellular matrix in ovarian follicle development.
Regulation of ovarian follicle development depends on endocrine- and paracrine-acting hormones, the 3-dimensional architecture of the follicle, and the physical rigidity of the surrounding tissue. These 3 forces are integrated throughout the life cycle of the follicle to ensure appropriate hormone secretion, differentiation of the somatic cells, and maturation of the oocyte. The process of in-f...
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The primary therapeutic goal for the oncology patient is survival. Recent advances in diagnoses and treatment of neoplasia have resulted in an ever-increasing number of patients being cured and resuming a normal life. However, recognized side effects of treatments used to eradicate malignancies are temporary or permanent sterility. The cryopreservation of ovarian tissue harvested before cancer ...
متن کاملP-121: Follicle Development in Vitrified Sheep Ovarian Tissue Transplanted to Immunodeficiencent Rat
Background: Ovarian cortex xenotransplantation into immunodeficient rats is a follicles protection method in rare animals and investigation on folliclogenesis. The aim of this study was to evaluate the effects of gonadotropin and erythropoietin (EPO) treatment on follicular development in the transplanted ovaries. Materials and Methods: Prepared sheep ovarian cortical strips were vitrified by c...
متن کاملEffect of extracellular matrix on testosterone production during in vitro culture of bovine testicular cells
Testosterone is believed to play a significant role in spermatogenesis, but its contribution to the process of spermatogenesis is not completely understood. Given that extracellular matrix (ECM) facilitates differentiation of spermatogonial stem cells (SSCs) during culture, the present study was conducted to elucidate whether testosterone contribute to the permissive effect of ECM on SSCs diffe...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2021
ISSN: 1472-6750
DOI: 10.1186/s12896-020-00658-3