Comparison of the transcriptomes and expression patterns of genes involved in key medicinal secondary metabolites from Astragalus membranaceus and Astragalus membranaceus var. mongholicus

Radix astragali, from the roots of Astragalus L. species, is regarded as an important traditional medicinal plant and has been widely used a Qi-Invigorating medicine for more than 2 000 years. Considering different metabolites or functional compounds astragali distinct underlying genetic information among two species important. Here, we compared root transcriptomes expression patterns genes involved in key secondary membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao A total 98 017 578 102 635 956 reads, including 294 560 92 494 416 high-quality were obtained A. membranaceus, respectively. In general, 73 785 (39.47%) 60 739 (34.93%) unigenes differentially expressed, indicating that had many homologous genes. comparison, carbon metabolism category contained most abundant mongholicus, it those found based on KEGG results. Genes may participate biosynthesis flavonoids, diterpenoids, triterpenoids, steroids, isoquinoline alkaloids, carotenoids, such CHS, F3H, GA3, CYPs, identified annotations. 5 227 101 transcription factors (TFs) Three TF categories showed twice numbers i.e., SOH1 (4:2), LIM (18:5), GRF (19:5), while MED7 (6:12). These results indicated pathways be synthesis active ingredients germplasm resources, data will provide valuable further utilization investigation these species.